TechNotes No. 7-2000
Binding of a plant lectin — horseradish peroxidase conjugate
Binding of lectins to immobilized saccharides and subsequent inhibition of such a binding event is often used for evaluating lectin inhibitors. The use of GlycoWell™ plates facilitates an ELISA assay involving the binding of lectins to the wells. This TechNote describes the binding of a 2–acetamido–2–deoxy–a–D–galactopyranoside recognizing lectin, Maclura pomifera1, to 2–acetamido–2–deoxy–a–D–galactopyranoside Glyco-Well™ plates.
The results demonstrate that a 2–acetamido–2–deoxy–a–D–galacto-pyranoside GlycoWell™ plate efficiently binds a 2–acetamido–2–deoxy–a–D–galactopyranoside-recognizing plant lectin, Maclura pomifera (Figure 1). Thus, GlycoWell plates are suitable for detecting carbohydrate binding properties of proteins.
|Figure 1. A Maclura pomifera-horseradish peroxidase conjugate binds to 2–acetamido–2–deoxy–a–D–galacto-pyranoside GlycoWell™ plates (SW-01-001), but not to a GlycoWell™ plate devoid of saccharide (SW-00-001).|